CHROMATOGRAPHIC AND COMPUTATIONAL APPROACHES TO THE PHARMACOKINETIC PROFILE OF PIROXICAM AND ITS SENSITIVE ASSAY IN RABBIT PLASMA TAVŞAN PLAZMASINDA PİROKSİKAMİN FARMAKOKİNETİK PROFİLİNE VE HASSAS ANALİZİNE YÖNELİK KROMATOGRAFİK VE HESAPLAMALI YAKLAŞIMLAR

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Aksoy O. A., Taskin-Tok T., Cotaoglu M. Z., Bakirhan N. K., Kocak E., Göksel B. A., ...Daha Fazla

Ankara Universitesi Eczacilik Fakultesi Dergisi, cilt.50, sa.2, ss.350-361, 2026 (Scopus, TRDizin) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 50 Sayı: 2
  • Basım Tarihi: 2026
  • Doi Numarası: 10.33483/jfpau.1766558
  • Dergi Adı: Ankara Universitesi Eczacilik Fakultesi Dergisi
  • Derginin Tarandığı İndeksler: Scopus, Central & Eastern European Academic Source (CEEAS), TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.350-361
  • Anahtar Kelimeler: ADMET, chromatography, DFT calculation, pharmacokinetic analysis, piroxicam
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Ankara Üniversitesi Adresli: Evet

Özet

Objective: A simple, rapid, sensitive, and cost-effective high-performance liquid chromatographic (HPLC) method was developed for the pharmacokinetic study and determination of Piroxicam. Material and Method: The chromatographic separation was performed with a Agilent ZORBAX reversed-phase C18 column (250 mm × 4,6 mm ID × 5 μm). Mobile phase composition was 0.04 M KH2PO4: acetonitrile:methanol (50:40:10) at pH 3.8. The flow rate was adjusted as 0.9 mL/min. Result and Discussion: An isocratic method was used for the analysis. The UV detector was set at 360 nm, and a retention time of 7 minutes was obtained for piroxicam. The method showed linear performance over the concentration range of 0.1–4 mg/l.