Antioxidantactivities of oleuropeinand the aqueous extracts of Olea europaea L. varieties growing in Turkey Türkiye'de yetişen Olea europaea L. varyetelerinin sulu ekstrelerinin ve Oleuropein'in antioksidan aktiviteleri

Altinyay Ç., Güvenç A., ALTUN M. L.

Turkish Journal of Pharmaceutical Sciences, cilt.8, sa.1, ss.23-30, 2011 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 1
  • Basım Tarihi: 2011
  • Dergi Adı: Turkish Journal of Pharmaceutical Sciences
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.23-30
  • Anahtar Kelimeler: Antioxidant activity, DPPH, Lipid peroxidation, Olea europaea
  • Ankara Üniversitesi Adresli: Evet

Özet

The genus Olea L. (Oleaceae) is represented by 67 species in the world. Two varieties of Olea europaea L. (Olea europaea L. var. europaea and Olea europaea var. sylvestris (Miller) Lehr.) are growing in Turkey and are found mostly in the West and South Anatolia. In this study, antioxidant activity of the aqueous extracts obtained from the leaves and branches of O. europaea and oleuropein were investigated. The antioxidant activities were studied by two different techniques: Qualitative DPPH• (1,1-diphenyl-2-picrylhydrazyl radical) assay to detect the free radical scavenging activity and the thiobarbituric acid (TBA)-assay to detect liposome lipid peroxidation. The lipid peroxidation was initiated in liposomes obtained from bovine brain extracts by addition of ascorbic acid and iron source and was measured spectrophotometrically with TBA test. The highest activity was observed with the extract of O. europaea var. sylvestris leaves with an IC50 value of 0.29 μg/ml where propil gallat (IC50=0.21±0.01) was used as the positive control.