Short-Term Preservation of Grass Carp (Ctenopharyngodon idella) Eggs: Effect of Extenders and Storage Duration

Bozkurt Y., Ogretmen F., SEÇER F. S.

ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH, cilt.62, sa.3, ss.189-194, 2010 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 62 Sayı: 3
  • Basım Tarihi: 2010
  • Dergi Adı: ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.189-194
  • Anahtar Kelimeler: eggs, extender, glucose solution, ovarian fluid, cold storage, fertilization, grass carp, ONCORHYNCHUS-MYKISS, SALMON, FERTILIZATION, TEMPERATURE, SURVIVAL, SPERM, OVA
  • Ankara Üniversitesi Adresli: Evet

Özet

The short-term effects of extenders and storage duration on fertilization, eyeing, and hatching rates in short-term stored grass carp eggs was evaluated. Eggs were kept in one of two artificial extenders (Ringer and 300 mM glucose solutions) or in ovarian fluid in 10-cm petri dishes. About 4 ml of artificial extender or coeolomic fluid was added to each petri dish and the dishes were transferred to a dark humid refrigerator for storage at 4 C for 120, 240, 360, or 480 min. Eggs were fertilized by adding 200 mu l milt to each petri dish. The fertilization rate was determined at the gastrula stage. The eyeing rate was defined as the number of eyed eggs divided by the initial number of eggs used for fertilization. The highest fertilization rate (93%) was attained in coelomic fluid stored for 120 min. The highest eyeing rates were in eggs kept in ovarian fluid for 120 or 240 min. The glucose extender was best for longer storage (360 and 480 min). The highest hatching rate was obtained with ovarian fluid at 240 min but the glucose extender was best for storage at 120, 360, and 480 min. Results indicate that glucose solution is the best extender for short-term (2-4 h) storage of grass carp eggs.