Ultrasound-assisted electromembrane extraction of clonazepam from plasma and determination using capillary electrophoresis

Seyfinejad B., Ozkan S. A., Jouyban A.

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, cilt.1181, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1181
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.jchromb.2021.122928
  • Dergi Adı: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Boundary layer, Central composite design, Interfacial tension, Mass transfer, Response surface methodology, Sonication, REINFORCED HOLLOW-FIBER, BASIC DRUGS, LIQUID-MEMBRANES, ACIDIC DRUGS, BREAST-MILK, MICROEXTRACTION, SURFACTANT, URINE, QUANTIFICATION, ENHANCEMENT
  • Ankara Üniversitesi Adresli: Evet

Özet

© 2021 Elsevier B.V.In this work, ultrasound-assisted electromembrane extraction (UA-EME) coupled with capillary electrophoresis (CE) and diode array detection (DAD) was developed for the determination of clonazepam from plasma samples. A comparative study was carried out between conventional EME and UA-EME methods to investigate the influence of the ultrasound waves on the extraction efficiency. The central composite design was used for the optimization of the variables affecting these methods to achieve the best extraction efficiency. Under optimal extraction conditions, the UA-EME provided better extraction recovery in a shorter time (58% in 13 min) than the EME method (42% in 30 min). Ultrasound reduces the extraction time and increased recovery by reducing the thickness of the barrier layer. In addition, this method provided a higher pre-concentration factor (2 0 3) and a lower limit of detection (3 ng mL−1) with good repeatability (RSDs were less than 10.11%).