Akademik Veri Yönetim Sistemi
33rd International Symposium on Pharmaceutical and Biomedical Analysis, Ankara, Türkiye, 2 - 06 Haziran 2023, ss.201-202
Efavirenz is a non-nucleoside reverse transcriptase inhibitor primarily used for treating human immunodeficiency virus and against colorectal, glioblastoma, and pancreatic cancers. Lately, a growing approach has been that approved drugs have been tested for additional purposes.
The construction of DNA nanobiosensor based on amine-functionalized multi-walled carbon nanotubes is fabricated for Efavirenz-DNA interaction. The functionalized multi-walled carbon nanotube is characterized by SEM, FTIR, Raman Spectroscopy and XRD techniques. The experimental conditions are optimized for nanobiosensor such as dropping volume of nanomaterial suspension, activation of nanosensor and concentration of ct-dsDNA. The experimental measurements were performed by differential pulse voltammetry using a glassy carbon electrode. Thanks to the amine-functionalized multi-walled carbon nanotubes, the peak currents of dsDNA were increased and the peak potentials of Efavirenz have shifted to the less positive potential. In addition, the interaction mechanism between dsDNA and Efavirenz was investigated using bare glassy carbon electrodes at different times, various concentrations and different physiological conditions.