Akademik Veri Yönetim Sistemi
Hacettepe University Journal of the Faculty of Pharmacy, cilt.36, sa.2, ss.135-149, 2016 (Scopus)
© 2016, Hacettepe University, Faculty of Pharmacy. All rights reserved.Ultra-high-performance liquid chromatographic (UPLC) data obtained from photodiode array (PDA) detection was processed by the PCR and PLS algorithms for the simultaneous quantitative resolution of Ascorbic Acid (AA), Paracetamol (PAR), Aspirin (ASP) in a commercial formulation. Principle component regression (PCR) and partial least squares (PLS) were applied to the peak area ratio of the analytes/internal standard at multi-wavelength PDA detector responses. The combined use of UPLC and chemometric calibration techniques was denoted UPLC- PCR and UPLC-PLS. For the comparison purpose, the UPLC method was used for the confirmation of the results obtained from combined UPLC-chemometric calibration techniques. A good chromatographic separation between drugs and internal standard (IS) was achieved using a Waters ACQUITY UPLC BEH Phenyl (100 mm x 1.0 mm, i.d., 1.7 μm) column and a mobile phase consisting of 0.1 M CH3COOH and methanol (75:25, v/v). The multi-wavelength PDA detection for Ascorbic Acid (AA), Paracetamol (PAR), Aspirin (ASP) was accomplished by measuring the peak area at the wavelength set corresponding to 245, 250, 255.0, 260.0, 265, 270, 275 and 280 nm. The proposed UPLC-PCR and UPLC-PLS approaches were validated by using the synthetic mixtures, and inter-day and intra-day experiments. The proposed methods were successfully applied to commercial samples containing the analyzed drugs.