PCR Detection of Coxiella burnetii in Fetal Abomasal Contents of Ruminants

Gunaydin E., MÜŞTAK H. K., SAREYYÜPOĞLU B., Ata Z.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.21, sa.1, ss.69-73, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.9775/kvfd.2014.11729
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.69-73
  • Anahtar Kelimeler: Coxiella burnetii, Fetal abomasal contents, Trans-PCR, REAL-TIME PCR, POLYMERASE-CHAIN-REACTION, Q-FEVER, DAIRY-CATTLE, NESTED PCR, TURKEY, ABORTIONS, MILK, INFECTION, DIAGNOSIS
  • Ankara Üniversitesi Adresli: Evet

Özet

This study was carried out on abomasal contents of aborted fetuses of 102 cattles, 45 sheep and 5 goats sent to the laboratory from Central Anatolia, and Black Sea Region of Turkey to investigate the presence Coxiella burnetii (C. burnetii) by trans-PCR. Out of 152 abomasal contents, 11 (7.23%) were detected to contain C. burnetii DNA. Four (3.92%) out of 102 cattle, 5 (11.11%) out of 45 sheep, and 2 (40%) out of 5 goat abomasal contents harbored C. burnetii DNA. As a result, PCR detection of C. burnetii DNA in fetal abomasum contents is a significant finding of a possible C. burnetii related abortion.