Antioxidant activity and phytochemical analysis of Alchemilla persica Rothm

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ERGENE B., BAHADIR ACIKARA Ö., BAKAR ATEŞ F., SALTAN İŞCAN H. G., Nebioǧlu S.

Ankara Universitesi Eczacilik Fakultesi Dergisi, cilt.39, sa.2, ss.145-154, 2010 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1501/eczfak_0000000560
  • Dergi Adı: Ankara Universitesi Eczacilik Fakultesi Dergisi
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.145-154
  • Anahtar Kelimeler: Alchemilla persica, Antioxidant activity, DPPH, MDA, Rosaceae
  • Ankara Üniversitesi Adresli: Evet

Özet

In present study, the extracts prepared using aerial parts and roots of Alchemilla persica Rothm. were evaluated for their antioxidant activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay and measurement of malondialdehyde (MDA) levels. HPLC analyses of the extracts were also performed using some phenolic acid and flavonoid standards. The hydro-methanolic extract of the aerial parts was found to possess significant antioxidant activity in both assays. IC50 values for the aerial parts and roots are determined as 0.055 M and 0.151 M respectively against DPPH radical. In TBARS assay, the MDA level of the aerial parts was found to be 5.9 nmol/ml, while 19.08 nmol/ml for the root extracts.