Comparative Proteomic Analysis of Salmonella Typhimurium LT2 and Its hisG Gene Inactivated Mutant

Erdogan I., AKÇELİK N., Akcelik M.

MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY, vol.30, no.1, pp.48-56, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 1
  • Publication Date: 2015
  • Doi Number: 10.3103/s0891416815010024
  • Journal Name: MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.48-56
  • Keywords: Salmonella, proteomics, hisG, SURVIVAL STRATEGIES, PROTEINS, ENTERICA, BIOSYNTHESIS, MACROPHAGE, EXPRESSION, REPRESSION, MUTATIONS, ADENINE
  • Ankara University Affiliated: Yes

Abstract

A hisG gene, encoding ATP phosphoribosyl phosphothansferase, insertion mutation was found to effected the production of four different proteins in Salmonella Typhimurium LT2 as a results of comparative analysis of two-dimensional analysis of hisG mutant (Delta his87) and the conrol strain (LT2). MALDI-TOF analysis of these protein spots which were expresed in S. Typhimurium LT2 but not its mutant Delta his87, showed that these proteins were; GMP synthase, arginine-tRNA ligase (Arginyl-tRNA synthetase), SopE and SifA. All of these proteins are virulence-associated proteins in Salmonella. Thus we have concluded that the proper function of histidine operon is very crucial for virulence of S. Typhimurium.