Comparative Proteomic Analysis of Salmonella Typhimurium LT2 and Its hisG Gene Inactivated Mutant

Erdogan I., AKÇELİK N., Akcelik M.

MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY, cilt.30, sa.1, ss.48-56, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.3103/s0891416815010024
  • Dergi Adı: MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.48-56
  • Anahtar Kelimeler: Salmonella, proteomics, hisG, SURVIVAL STRATEGIES, PROTEINS, ENTERICA, BIOSYNTHESIS, MACROPHAGE, EXPRESSION, REPRESSION, MUTATIONS, ADENINE
  • Ankara Üniversitesi Adresli: Evet

Özet

A hisG gene, encoding ATP phosphoribosyl phosphothansferase, insertion mutation was found to effected the production of four different proteins in Salmonella Typhimurium LT2 as a results of comparative analysis of two-dimensional analysis of hisG mutant (Delta his87) and the conrol strain (LT2). MALDI-TOF analysis of these protein spots which were expresed in S. Typhimurium LT2 but not its mutant Delta his87, showed that these proteins were; GMP synthase, arginine-tRNA ligase (Arginyl-tRNA synthetase), SopE and SifA. All of these proteins are virulence-associated proteins in Salmonella. Thus we have concluded that the proper function of histidine operon is very crucial for virulence of S. Typhimurium.