A Novel DNA Biosensor Based on a Pencil Graphite Electrode Modified with Polypyrrole/Functionalized Multiwalled Carbon Nanotubes for Determination of 6-Mercaptopurine Anticancer Drug

Karimi-Maleh H., Tahernejad-Javazmi F., Atar N., Lutfi M. L., Gupta V. K., Ensafi A. A.

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, cilt.54, sa.14, ss.3634-3639, 2015 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 54 Sayı: 14
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1021/ie504438z
  • Dergi Adı: INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.3634-3639
  • Ankara Üniversitesi Adresli: Hayır

Özet

A novel and sensitive biosensor employing immobilized DNA on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for the determination of 6-mercaptopurine (6-MP) is presented. In the first step, we modified the pencil graphite surface with polypyrrole and functionalized multiwalled carbon nanotubes (MWCNT/COOH). The developed electrode was characterized by scanning electron microscopy, atomic force microscopy, reflectionabsorption infrared spectroscopy, X-ray photoelectron spectroscopy, and electrochemical impedance spectroscopy. In the other step, we used decreases in the oxidation responses of guanine and adenine as a sign of the interaction of 6-MP with salmon sperm double-stranded DNA using differential pulse voltammetry. The signal of guanine oxidation was linear with respect to the 6-MP concentration in the range of 0.2-100 mu mol L-1 with a detection limit of 0.08 mu mol L-1. The modified electrode was utilized for the determination of 6-MP in real samples.