Application of derivative-differential UV spectrophotometry and ratio derivative spectrophotometric determination of mephenoxalone and acetaminophen in combined tablet preparation

Erk N.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, cilt.21, sa.2, ss.429-437, 1999 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 2
  • Basım Tarihi: 1999
  • Doi Numarası: 10.1016/s0731-7085(99)00157-0
  • Dergi Adı: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.429-437
  • Anahtar Kelimeler: mephenoxalone, acetaminophen, pharmaceutical formulation, first derivative-differential spectrophotometry, ratio derivative spectrophotometry, DOSAGE FORMS, PARACETAMOL, MIXTURES, ASSAY
  • Ankara Üniversitesi Adresli: Evet

Özet

A method is presented for the direct determination of mephenoxalone and acetaminophen in combined pharmaceutical dosage forms without prior separation. The first method, derivative-differential spectrophotometry, comprised of measurement of the difference absorptivities derivatized in the first order (Delta D-1) of a tablet extract in 0.1 N NaOH relative to that of an equimolar solution in methanol at wavelengths of 289.6 and 252.6 nm respectively. The second method is based on ratio derivative spectrophotometry. The amplitudes in the first derivative of the ratio spectra at 233.5 and 288.9 nm were selected to determine mephenoxalone and acetaminophen in the mixture. The proposed methods, which give thoroughly comparable data, are simple and rapid, and allow one to obtain precise and accurate results. (C) 1999 Elsevier Science B.V. All rights reserved.