INTERACTION BETWEEN ELTROMBOPAG AND HUMAN SERUM ALBUMIN: FLUORESCENCE AND ELECTROCHEMICAL STUDIES ELTROMBOPAG VE İNSAN SERUM ALBÜMİNİ ARASINDAKİ ETKİLEŞİM: FLORESANS VE ELEKTROKİMYASAL ÇALIŞMALAR

YENCE M., Cetinkaya A., Bellur Atici E., ÖZKAN S. A.

Ankara Universitesi Eczacilik Fakultesi Dergisi, vol.49, no.3, pp.859-869, 2025 (Scopus, TRDizin) identifier identifier

  • Publication Type: Article / Article
  • Volume: 49 Issue: 3
  • Publication Date: 2025
  • Doi Number: 10.33483/jfpau.1663961
  • Journal Name: Ankara Universitesi Eczacilik Fakultesi Dergisi
  • Journal Indexes: Scopus, Central & Eastern European Academic Source (CEEAS), EMBASE, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.859-869
  • Keywords: Differential pulse voltammetry, drug protein interaction, eltrombopag, fluorescence spectroscopy, human serum albumin
  • Ankara University Affiliated: Yes

Abstract

Objective: This research investigated the interaction between Eltrombopag (EB) and Human Serum Albumin (HSA) by fluorescence spectroscopy and voltammetry while explaining the quenching mechanism. Material and Method: The electrochemical studies were conducted in an acetate buffer solution (AB) at pH 4 by differential pulse voltammetry (DPV). For the fluorescence studies, EB, HSA, and a mixture of EB and HSA solutions were designed with a pH of 4.7, AB containing 20% DMSO. Result and Discussion: The interaction between EB and HSA was examined by fluorescence and electrochemical titrations, which showed a quenching effect and peak shifting. Fluorescence titrations indicate that EB’s HSA quenching process is static and has a hypsochromic shift. Analysis of thermodynamic parameters and higher binding constants concluded a strong and spontaneous interaction. Electrochemical titrations show the intercalation of EB into HSA.