INTERACTION BETWEEN ELTROMBOPAG AND HUMAN SERUM ALBUMIN: FLUORESCENCE AND ELECTROCHEMICAL STUDIES ELTROMBOPAG VE İNSAN SERUM ALBÜMİNİ ARASINDAKİ ETKİLEŞİM: FLORESANS VE ELEKTROKİMYASAL ÇALIŞMALAR
Ankara Universitesi Eczacilik Fakultesi Dergisi, cilt.49, sa.3, ss.859-869, 2025 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 49 Sayı: 3
- Basım Tarihi: 2025
- Doi Numarası: 10.33483/jfpau.1663961
- Dergi Adı: Ankara Universitesi Eczacilik Fakultesi Dergisi
- Derginin Tarandığı İndeksler: Scopus, Central & Eastern European Academic Source (CEEAS), EMBASE, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.859-869
- Anahtar Kelimeler: Differential pulse voltammetry, drug protein interaction, eltrombopag, fluorescence spectroscopy, human serum albumin
- Ankara Üniversitesi Adresli: Evet
Özet
Objective: This research investigated the interaction between Eltrombopag (EB) and Human Serum Albumin (HSA) by fluorescence spectroscopy and voltammetry while explaining the quenching mechanism. Material and Method: The electrochemical studies were conducted in an acetate buffer solution (AB) at pH 4 by differential pulse voltammetry (DPV). For the fluorescence studies, EB, HSA, and a mixture of EB and HSA solutions were designed with a pH of 4.7, AB containing 20% DMSO. Result and Discussion: The interaction between EB and HSA was examined by fluorescence and electrochemical titrations, which showed a quenching effect and peak shifting. Fluorescence titrations indicate that EB’s HSA quenching process is static and has a hypsochromic shift. Analysis of thermodynamic parameters and higher binding constants concluded a strong and spontaneous interaction. Electrochemical titrations show the intercalation of EB into HSA.