Impaired endothelium-dependent and neurogenic relaxation of corpus cavernosum from diabetic rats: improvement with L-arginine

Gur S., Ozturk B., KARAHAN S. T.

UROLOGICAL RESEARCH, cilt.28, sa.1, ss.14-19, 2000 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 1
  • Basım Tarihi: 2000
  • Doi Numarası: 10.1007/s002400050003
  • Dergi Adı: UROLOGICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.14-19
  • Anahtar Kelimeler: acetylcholine, diabetes mellitus, L-arginine, corpora cavernosa, NITRIC-OXIDE SYNTHASE, NADPH-DIAPHORASE, AMINO-ACIDS, NEURONS, PENIS, BRAIN
  • Ankara Üniversitesi Adresli: Evet

Özet

This slurry describes the relaxant response to acetylcholine, electrical field stimulation and sodium nitroprusside after contraction by phenylephrine (10(-5) M) in corpus cavernosum from control and diabetic rats. The response to acetylcholine (10(-9)-10(-3) M) and electrical field stimulation (0.5-64 Hz) is decreased and can be restored by the addition of nitric oxide synthatase substrate, L-arginine(10(-5) M). The response to sodium nitroprusside is not changed in diabetic rats compared to control rats. NADPH-diaphorase staining was enhanced in a diabetic preparation compared to control preparations. The findings suggest a role for the depletion of L-arginine in diabetes mellitus. The enhanced NADPH-diaphorase staining may be due to a deficiency of NOS substrate L-arginine in the endothelium and nerves of diabetic tissues.