Isolation of Salmonella spp. from poultry enterprises in Ankara region

Ata Z., Aydin N.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.55, no.3, pp.161-166, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 3
  • Publication Date: 2008
  • Journal Name: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.161-166
  • Keywords: Egg, faeces, isolation, PCR, Salmonella, POLYMERASE CHAIN-REACTION, RAPID DETECTION, ENTERITIDIS, SAMPLES, DNA, EGGS
  • Ankara University Affiliated: Yes

Abstract

In this Study, the aim was to diagnose the Salmonellae in chicken faeces and eggs with both classical techniques and PCR.For this aim, 50 commercial layer poultry flocks in Ankara were visited and 20 cloacal swabs and 10 egg samples were taken from each flock. Twenty cloacal swaps taken from each flock were divided into two groups in which 10 of them were accepted and evaluated as one sample. A total of 500 eggs was accepted as 50 samples and evaluated by means of the presence of Salmonellae. At the end of the study, PCR and classical culture methods carried out after selective enrichment revealed that Salmonellae were identified oil the genus level from 6 (12%) out of 50 layer poultry flocks and from 6 (6%) individual cloacal swabs out of 100 samples. In this study, it has been shown that Salmonellae could be diagnosed with PCR method and this method could be used in routine diagnosis.