EFFICIENT AND SENSITIVE ANALYSIS OF BIOMOLECULAR INTERACTIONS BETWEEN PALBOCICLIB AND HUMAN SERUM ALBUMIN USING FLUORESCENCE QUENCHING MECHANISM PALBOSİKLİB VE İNSAN SERUM ALBÜMİN ARASINDAKI BİYOMOLEKÜLER ETKİLEŞİMLERİN FLORESAN SÖNÜMLEME MEKANİZMASI KULLANILARAK ETKİLİ VE HASSAS BİR ŞEKİLDE ANALİZ EDİLMESİ

Cetinkaya A., YENCE M., Atici E. B., ÖZKAN S. A.

Ankara Universitesi Eczacilik Fakultesi Dergisi, cilt.50, sa.1, ss.144-154, 2026 (Scopus, TRDizin) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 50 Sayı: 1
  • Basım Tarihi: 2026
  • Doi Numarası: 10.33483/jfpau.1763144
  • Dergi Adı: Ankara Universitesi Eczacilik Fakultesi Dergisi
  • Derginin Tarandığı İndeksler: Scopus, Central & Eastern European Academic Source (CEEAS), TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.144-154
  • Anahtar Kelimeler: Drug-protein interaction, fluorescence spectroscopy, human serum albumin, palbociclib, quenching mechanism
  • Ankara Üniversitesi Adresli: Evet

Özet

Objective: In this research, the interaction between Palbociclib (PC) and Human Serum Albumin (HSA) was examined using fluorescence spectroscopy, and the quenching mechanism was elucidated. Material and Method: HSA and PC stock solutions were prepared using 0.1 M pH 7.4 phosphate buffer solution and DMSO, respectively. In the protein-drug interaction experiment, the HSA concentration was fixed at 20 µM, and drug concentrations for the PC-HSA system were used between 0 and 40 µM. Result and Discussion: The interaction between PC and HSA was investigated at various temperatures using spectroscopic techniques. To comprehend the interaction process, experiments were carried out at different physiological pH levels and temperatures. Other parameters, including the number of binding sites, binding constants, and quenching constants, were calculated using the proper formulas. The van't Hoff equation was used to determine thermodynamic parameters such as entropy, Gibbs, and enthalpy at different temperatures.