Material and Method: The In-chemico method known as Direct Peptide Reactivity Assay (DPRA) is used. The test's objective is to aid in the evaluation of chemicals' potential to cause skin sensitivity. An essential process in allergies is thought to be haptenization, in which a low molecular weight substance called haptens creates a covalent bond with proteins in the skin. Therefore, the evaluation of the skin sensitization potential of chemicals is based on data from peptide reactivity tests, such as DPRA. Our study's goal was to build up the DPRA in accordance with the guidelines by using compounds known for their sensitization potency.

Result and Discussion: Our DPRA study's susceptibility classification of cosmetic compounds produced results that agreed with those of earlier in-vivo and ex-vivo tests on these substances. In order to demonstrate that, the DPRA approach can distinguish between irritation and allergy, sodium dodecyl sulfate (SDS) was used in this study, which has a strong irritation feature. A more accurate estimate of the sensitization potential can be achieved by integrating the best results from two or more methods. The weight-of-evidence technique should be used in conjunction with information from various sources rather than using it as a stand-alone strategy. As a result, a differentiation between sensitizing and non-sensitizing chemicals can be understood.

Keywords: Allergic contact dermatitis potency, DPRA, hapten, in-chemico