Akademik Veri Yönetim Sistemi
5th Eurasia Biochemical Approaches & Technologies (EBAT) Congress, Antalya, Türkiye, 2 - 05 Kasım 2023, ss.88
Cardiopulmonary bypass (CPB) is the temporary suspension of the heart and lungs to permit cardiac, vascular, or thoracic surgery in a safe, immobile, bloodless, and regulated environment. The goal of utilizing hypothermia is to provide some degree organ protection during CPB.1 The significance of hypothermia is difficult to comprehend since its effects are mediated by various systemic and cellular pathways.2 This study was comprised of 16 patients with (32-35°C) mild hypothermia (MIH) and 16 patients with (26-31°C) moderate hypothermia (MOH) following open heart surgery with CPB (n=32). Plasma samples were collected before surgery (T0), at the lowest intraoperative body temperature (T1), where after CPB was stopped and the patient reached normothermic (36.5°C) body (rectal) temperature (T2). Metabolomic profiles of both groups (moderate and mild hypothermia) were performed using GC-MS and LC-qTOF-MS technologies. Metabolomic analysis showed different metabolites to be altered in both hypothermia groups (p<0.05). T0was set as the preoperative control point to determine individual differences within the groups. It was also determined that phenylalanine and D-glucose-6-phosphoric acid showed change of each group between sampling time points T1and T2. Comparing the hypothermia groups independent of time points, Dehidroisoandrosterone Sulfate, Inosine, and N-Ethylglycine 2-Hydroxybuthyric acid metabolite levels increased in MIH, while L-valine, N,N-Dimethyl-Safingol, and N-octadecanoyl-sphinganine increased in the MOH group. It was determined that the phenylalanine, tyrosine, and tryptophan biosynthesis pathways caused differentiation between the MOH and MIH groups. Consequently, the metabolomic profile in both groups may have the potential for optimization of temperature strategies in CPB.
Acknowledgement: This study is supported by TÜBİTAK (the Scientific and Technological Research Council of Turkey), project number: 123S448. Approval was obtained from Ankara University Faculty of Medicine Human Research Ethics Committee (dated January 13, 2022; number İ01-12-22).
References:
1. Falter F, Perrino AC, Perrino AC and Baker RA. (2022). Cardiopulmonary Bypass. 3th ed. UK: Cambridge University Press.
2. R. Gocoł, D. Hudziak, J. Bis, K. Mendrala, Ł. Morkisz and T. Darocha, The Role of Deep Hypothermia in Cardiac Surgery. Int. J. Environ. Res. Public Health. 2021, 18, 7061.