Evaluation of increased incubation temperature and cefixime-tellurite treatment for the isolation of Escherichia coli O157 : H7 from minced beef
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, vol.87, no.1-2, pp.29-34, 2003 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 87 Issue: 1-2
- Publication Date: 2003
- Doi Number: 10.1016/s0168-1605(03)00045-x
- Journal Name: INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.29-34
- Keywords: Escherichia coli O157 : H7, cohabitant flora, increased temperature, cefiximie-tellurite, HEMORRHAGIC COLITIS, RADISH SPROUTS, GROUND-BEEF, GROWTH, ENRICHMENT, SEROTYPE, O157H7, H7
- Ankara University Affiliated: No
Abstract
To improve enrichment and isolation of Echerichia coli O157:H7, this study evaluated increased incubation temperature and cefixime-tellurite (CT) on five strains of each of the following bacteria, E. coli, Hafnia alvei, Enterobacter spp., Citrobacter freundii and E. coli O157:H7, and two strains of E. coli O157:nH7. These were grown in pure culture in LST broth with varying cefixime-tellurite concentrations. A range of incubation temperatures from 37 to 46 degreesC was investigated for the inhibition of cohabitant microorganisms. Minced beef, spiked with E. coli O157:H7 and cohabitant microorgamsims was investigated. Increased incubation temperature (42 degreesC) and treatment with half of the prescribed amount of cefixime-tellurite by BAM for SMAC agar in enrichment step were the most effective in selectively growing E. coli O157:H7. The results show that E. coli O157:H7 is more resistant to these two conditions than the other cohabitant bacteria. (C) 2003 Elsevier Science B.V. All rights reserved.