The Virulence Characteristics of Pseudomonas spp. and Vibrio spp. isolated from cultured fish species

Duman M., Aydın F. G., Satıcıoğlu İ. B.

World Aquatic Health Conference 2022, Pretoria, Güney Afrika, 4 - 07 Aralık 2022

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Pretoria
  • Basıldığı Ülke: Güney Afrika
  • Ankara Üniversitesi Adresli: Evet

Özet

 

Rainbow trout is one of the highest cultured fish species because of its resistance against disease, variable water quality and stress factors by comparison with other cultured species.

Among all of European countries, Turkey is the biggest rainbow trout (portion-size) producer, according to the FEAP report (FEAP, 2018). Pseudomonas and Vibrio species have been described as one of the most common bacterial infectious agents of cultured fish and have been reported to cause stress-related diseases in freshwater fish (López et al., 2012; Derome et al., 2016) which limits the fish production in Turkey (Ozturk and Altinok, 2014; Duman et al., 2018;Saticioglu et al., 2018). In the present study, we aimed that virulence characterization of Pseudomonas spp. and Vibrio spp. isolated from cultured fish species mainly rainbow trout, sea bream and sea bass in Turkey. A total of 93 Pseudomonas strains tree groups, five subgroups and 24 species and 54 Vibrio strains includes 7 species which were described before by Duman et al. (2022 and 2023). To the genomic virulence characterization of these isolates, Pseudomonas isolates were analyzed for 19 different virulence genes and Vibrio isolates were analyzed for 21 virulence genes which were commonly reported genes by polymerase chain reaction (PCR). The target virulence genes amplified using optimum melting temperature and standardized PCR master mix in thermal cycler and amplicons were visualized in agarose gel by automatized gel imaging system. The fliC-CW virulence gene encodes flagellin in Pseudomonas strains and serine protease gene encodes the extracellular products (ECP) of Vibrio species was reported to be lethal to fish were most common detected genes. For Pseudomonas strains, toxA, exoT, lasI and pvdA were the other detected genes and for Vibrio strains, Vah1 F-R, Vah2 F-R, Vah4 F-R, Vah5 F-R, Zot F-R, VVH F-R, Collagenase F-R, tcpA F-R, chiA, gyrB, vhpA, vpsR, nanH, vhh, tlhr2, luxR, toxRVc, flaC and VP-PA were also detected. As a conclusion, while five virulence genes were detected in 93 Pseudomonas isolates, 22 virulence genes were found in 54 Vibrio isolates. Our results showed that Vibrio species could be more pathogenic bacteria species for cultured fish, especially rainbow trout, sea bream and sea bass, than Pseudomonas species.