Development of a beta-cyclodextrin-modified gold nanoparticle-assisted electromembrane extraction method followed by capillary electrophoresis for methadone determination in plasma

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Hoseininezhad-Namin M. S., ÖZKAN S. A., Rahimpour E., Jouyban A.

RSC ADVANCES, vol.12, no.52, pp.33936-33944, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 52
  • Publication Date: 2022
  • Doi Number: 10.1039/d2ra06419g
  • Journal Name: RSC ADVANCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Biotechnology Research Abstracts, Chemical Abstracts Core, Compendex, Metadex, Directory of Open Access Journals
  • Page Numbers: pp.33936-33944
  • Ankara University Affiliated: Yes


In this study, gold nanoparticles (AuNPs) modified with beta-cyclodextrin (beta-CD) were used to assist with electromembrane extraction (EME) and were coupled with capillary electrophoresis (CE) and ultraviolet (UV) detection (CE-UV) for the extraction and measurement of methadone from plasma samples. A beta-CD-modified AuNP-reinforced hollow fiber (HF) was utilized in this work. The beta-CD-modified AuNPs act as an absorbent and provide an extra pathway for the analyte extraction. For obtaining the effect of the presence of beta-CD-modified AuNPs in the HF pores, the extraction efficiency of the EME and beta-CD-modified AuNPs/EME techniques were compared. Different parameters influencing the extraction efficacy of the EME and beta-CD-modified AuNPs/EME methods were optimized. Optimal extractions were performed with 1-octanol as the organic solvent in the supported liquid membrane (SLM), with an applied voltage of 10 V as the driving force across the SLM, and with pH 7.0 in the donor solutions with a stirring speed of 1000 rpm after 20 min and 25 min for the beta-CD-modified AuNPs/EME and EME methods, respectively. Under optimal conditions, compared with the EME method, the beta-CD-modified AuNPs/EME method exhibited increased extraction efficacy in a short time. The beta-CD-modified AuNPs/EME technique demonstrated a lower limit of detection (5.0 ng mL(-1)), higher extraction recovery (68%), and a more optimal preconcentration factor (135). Furthermore, this method was successfully utilized for measuring methadone in real plasma samples.