Development of the electrochemical, spectroscopic and molecular docking approaches toward the investigation of interaction between DNA and anti-leukemic drug azacytidine

Nimal R., Unal D. N., Erkmen C., BOZAL PALABIYIK B., Siddiq M., EREN G., ...Daha Fazla

BIOELECTROCHEMISTRY, cilt.146, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 146
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.bioelechem.2022.108135
  • Dergi Adı: BIOELECTROCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Azacytidine, DNA, Interaction, Voltammetry, Spectroscopy, Binding, GLASSY-CARBON ELECTRODE, CALF-THYMUS DNA, ANTICANCER DRUG, LABEL-FREE, BINDING, METHYLATION, BIOSENSOR, FLUORESCENCE, AZACITIDINE, INHIBITION
  • Ankara Üniversitesi Adresli: Evet

Özet

This study examines the interaction between pyrimidine nucleoside analogue azacytidine, an anti-leukemic drug, and DNA by employing electrochemical, UV-vis spectroscopy, fluorescence spectroscopy and molecular docking techniques. In the electrochemical technique, azacytidine and dsDNA interaction was investigated in two different ways: (1) in solution and (2) with a biosensor using differential pulse voltammetry (DPV) at a glassy carbon electrode. The interaction between azacytidine and dsDNA at increasing interaction times was investigated in line with the changes in adenine and guanine oxidation signals. In addition, interaction studies of polyguanine-azacytidine and polyadenine-azacytidine were performed with DPV. The binding constant values were calculated as 2.420 x 10(4) M-1 and 3.266 x 10(4) M-1 at 25 degrees C using UV and fluorescence spectroscopy, respectively. In conclusion, based on electrochemical and spectroscopic methods as well as molecular docking studies, it was predicted that azacytidine can bind to dsDNA via groove binding.