Studies on the activities of drug metabolizing enzymes in lung and kidney microsomes of rat Siçan akci̇ǧeri̇ ve böbreǧi̇ mi̇krozomlarinda i̇laç metaboli̇ze eden enzi̇mleri̇n akti̇vi̇teleri̇ üzeri̇ne çalişmalar

EKE B.

Ankara Universitesi Eczacilik Fakultesi Dergisi, vol.32, no.1, pp.1-18, 2003 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 1
  • Publication Date: 2003
  • Journal Name: Ankara Universitesi Eczacilik Fakultesi Dergisi
  • Journal Indexes: Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.1-18
  • Keywords: Kidney, Lung, Microsomes, Monooxygenases, Rat
  • Ankara University Affiliated: Yes

Abstract

The optimum conditions (pH, protein amount, subtrate concentration and incubation time) of 7-ethoxyresorufin-O-deethylase(EROD), 7-pentoxyesorufin-O-deethylase(PROD), eryhtromycin-N-demethylase (ERND), coumarin 7-hydroxylase (Coh) and p-nitrophenol (PNP) activities in the lung and kidney of rat were determined in microsomes. In this study, it was found out that, different reaction conditions are required for these enzyme activities in rat lund and kidney microsomes.