Immobilization of glucose oxidase onto gelatin for biosensor construction

EMREGÜL E., Sungur S., Akbulut U.

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, vol.16, no.4, pp.505-519, 2005 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 16 Issue: 4
  • Publication Date: 2005
  • Doi Number: 10.1163/1568562053700138
  • Journal Name: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.505-519
  • Keywords: enzyme biosensor, glucose oxidase, gelatin, polyaniline, immobilization, ASPERGILLUS-NIGER, POLYANILINE, ENZYME, FILMS, CARBOXYMETHYLCELLULOSE, INVERTASE, UREASE
  • Ankara University Affiliated: Yes

Abstract

The properties of a glucose biosensor made by immobilization of glucose oxidase onto gelatin in a layer of electrochemically deposited polyaniline have been investigated. Glucose oxidase was immobilized within gelatin cross-links with chromium(III) acetate. The glucose oxidase biosensor was developed by forming a polyaniline-deposited electrode surface as support for the immobilized enzyme gel, in order to increase its durability. The polyaniline/gelatin/glucose oxidase biosensor has been characterized using chemical and electrochemical methods. Temperature, pH, cross-linking agent concentration, enzyme concentration, kinetic properties, reusability and the effect of electro-active compounds were among the parameters studied. The response time of the glucose oxidase biosensor is 90 s, the detection limit is below 1 mmol/dm(3) and the sensor can be used 20 times within a 2-month period without losing its stability.