Akademik Veri Yönetim Sistemi
PLANT BIOSYSTEMS, cilt.159, sa.6, ss.1419-1435, 2025 (SCI-Expanded, Scopus)
The genus Scutellaria, known as "kaside" in Turkey and "skullcap" globally, is primarily found in temperate regions and tropical mountains. This study examined the total aqueous methanolic extract and petroleum ether, ethyl acetate, and aqueous sub-extracts from the aerial parts of Scutellaria yildirimlii. Using HPLC, scutellarein, apigenin, and luteolin-7-O-glucoside were identified. Antioxidant activities were assessed via total antioxidant capacity and ferric-reducing antioxidant potential assays, showing significantly higher activity in all extracts compared to the control. The ethyl acetate extract, which showed the highest activity, underwent isolation studies and the flavonoid baicalin was identified using 1H-NMR and mass spectrometry. Baicalin and the petroleum ether extract demonstrated the strongest anti-proliferative effects in MTT assays. In addition, the xCELLience (Real Time Analyzer) device was used to obtain more sensitive, accurate, and valid results. In silico studies revealed baicalin's limited gastrointestinal absorption and poor blood-brain barrier permeation. Baicalin is predicted as a potential inhibitor of NADPH oxidase, a free radical scavenger, and an inhibitor of lipid peroxidation, targeting tyrosine-protein kinase FYN and multidrug resistance-associated protein 1, and possibly serine/threonine-protein kinase NEK6. This study provides the first isolation of baicalin from S. yildirimlii and highlights its significant antioxidant and anti-cancer potential, underscoring its medical importance.