Detailed electrochemical behavior and thermodynamic parameters of anticancer drug regorafenib and its sensitive electroanalytical assay in biological and pharmaceutical samples

Doulache M., Kaya S. I., Cetinkaya A., Bakirhan N. K., Trari M., Ozkan S. A.

Microchemical Journal, cilt.170, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 170
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.microc.2021.106717
  • Dergi Adı: Microchemical Journal
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Food Science & Technology Abstracts, Index Islamicus, Veterinary Science Database
  • Anahtar Kelimeler: Regorafenib, Multi-walled carbon nanotubes, Adsorptive stripping differential pulse, voltammetry, Drug analysis, GLASSY-CARBON ELECTRODE, LIQUID-CHROMATOGRAPHY, CYTOCHROME-C, NANOTUBES, METABOLITES, CANCER, PLASMA, SERUM
  • Ankara Üniversitesi Adresli: Evet

Özet

© 2021 Elsevier B.V.In this study, an electrochemical sensing platform was prepared to determine regorafenib on multi-walled carbon nanotubes (MWCNTs) modified glassy carbon electrodes (GCE) by adsorptive stripping differential pulse voltammetry (AdsDPV). The electrooxidation of regorafenib was an irreversible reaction and governed by an adsorption process that is pH-dependent. According to the oxidation mechanism study, two electrons were required for electrochemical oxidation of regorafenib in 0.1 M H2SO4. The influence of temperature was studied to evaluate the kinetic and thermodynamic parameters using the cyclic voltammetry method in different temperature values. Using adsorptive stripping mode, a significant improvement in the response signal of regorafenib was registered on the surface of MWCNTs/GCE as compared to bare GCE. MWCNTs/GCE sensor was successfully used for the determination of regorafenib in a linear range between 5 × 10-7 M and 2.5 × 10-5 M (R2 = 0.998) with a limit of detection and limit of quantification values of 2.08 × 10-8 M and 6.93 × 10-8 M in 0.1 M H2SO4 solution. Additionally, for the evaluation of the applicability of the developed nanomaterial-modified sensor platform, it was applied to human serum samples and pharmaceutical tablet dosage forms with good recovery results.