A rapid lateral flow stool antigen immunoassay and C-14-urea breath test for the diagnosis and eradication of Helicobacter pylori infection in children

KULOĞLU Z., KANSU TANCA A., TUNA KIRSAÇLIOĞLU C., Uestuendag G., Aysev D., ENSARİ A., ...More

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, vol.62, no.4, pp.351-356, 2008 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 62 Issue: 4
  • Publication Date: 2008
  • Doi Number: 10.1016/j.diagmicrobio.2008.07.006
  • Journal Name: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.351-356
  • Keywords: Children, Helicobacter pylori, Immunoassay, Immunochromatography, Stool antigen test, C-14 urea breath test, MONOCLONAL ENZYME-IMMUNOASSAY, C-14 UREA, ACCURACY, VALIDATION, GASTRITIS, AGE
  • Ankara University Affiliated: Yes

Abstract

Our aim was to evaluate diagnostic accuracy of rapid immunochromatographic stool antigen test (Rapid HpSA; LINEAR Chemical, Barcelona, Spain) and a practical low-dose C-14 urea breath test (UBT) (Heliprobe(TM)) test before and after eradication therapy. One hundred nine children with abdominal symptoms (age range, 5-17 years; mean, 12.1) underwent endoscopy, C-14-UBT, and Rapid HpSA. Patients were defined as Hp infected when histology was positive for Hp. Forty children (36.6%) were Hp infected. The sensitivity of Rapid HpSA and C-14-UBT was 65% and 92.5% (P = 0.0003), respectively; the specificity of Rapid HpSA and C-14-UBT was 92.3% and 85.5% (P = 0.180), respectively. After eradication therapy endoscopy, C-14-UBT and Rapid HpSA were repeated. The eradication rate was 70.5%. After eradication, the sensitivity of Rapid HpSA and C-14-UBT was 60% and 100%, respectively; the specificity of Rapid HpSA and C-14-UBT was 100%. C-14-UBT was more reliable than the Rapid HpSA test for the diagnosis and for confirming eradication of Hp infection. (C) 2008 Elsevier Inc. All rights reserved.