Osteogenic differentiation of MC3T3-E1 cells on different titanium surfaces

Hakki S. S., Bozkurt S. B., Hakki E. E., KORKUSUZ P., PURALI N., Koç N., ...More

Biomedical Materials, vol.7, no.4, 2012 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 4
  • Publication Date: 2012
  • Doi Number: 10.1088/1748-6041/7/4/045006
  • Journal Name: Biomedical Materials
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Ankara University Affiliated: Yes


mRNA expressions related to osteogenic differentiation of MC3T3-E1 cells on electro-polished smooth (S), sandblasted small-grit (SSG) and sandblasted large-grit (SLG) surfaces of titanium alloys were investigated in vitro. Gene expression profiles of cells were evaluated using the RT2 Profiler PCR microarray on day 7. Mineralizing tissue-associated proteins, differentiation factors and extracellular matrix enzymes mRNA expressions were measured using Q-PCR. SLG surface upregulated 23 genes over twofolds and downregulated 3 genes when compared to the S surface. In comparison to the SSG surface, at least a twofold increase in 25 genes was observed in the SLG surface. BSP, OCN, OPN, COL I and ALP mRNA expressions increased in the SLG group when compared to the S and the SSG groups. BMP-2, BMP-6 and TGF-β mRNA expressions increased in both the SSG and the SLG surfaces. MMP-2 and MMP-9 mRNA expressions increased as the surface roughness increased. This study demonstrated that surface roughness of titanium implants has a significant effect on cellular behavior and SLG surface apparently increased gene expressions related to osteogenesis when compared to the S and the SSG surfaces. © 2012 IOP Publishing Ltd.