Fabrication of poly(3,4-ethylenedioxythiophene)-iridium oxide nanocomposite based Tyrosinase biosensor for the dual detection of catechol and azinphos methyl

Erkmen C., KURBANOĞLU S., USLU B.

SENSORS AND ACTUATORS B-CHEMICAL, cilt.316, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 316
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.snb.2020.128121
  • Dergi Adı: SENSORS AND ACTUATORS B-CHEMICAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Analytical Abstracts, Biotechnology Research Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Anahtar Kelimeler: Catechol detection, Enzyme inhibition, Poly(3,4-ethylenedioxythiophene) nanoparticles, Iridium oxide, Pesticides, Tyrosinase, INHIBITION-BASED BIOSENSORS, ORGANOPHOSPHORUS PESTICIDES, POLYAMIDOAMINE DENDRIMER, AMPEROMETRIC BIOSENSOR, CONDUCTING POLYMERS, PHENOLIC-COMPOUNDS, ENZYME, ELECTRODE, NANOPARTICLES, WATER
  • Ankara Üniversitesi Adresli: Evet

Özet

A novel, sensitive and simple amperometric biosensor for the determination of catechol and azinphos methyl based on Tyrosinase enzyme inhibition has been developed. The biosensor was prepared from iridium (IV) oxide, Poly (3,4 ethylenedioxythiophene) nanocomposite, and Tyrosinase immobilized by crosslinking with glutaraldehyde in this nanocomposite platform. Biosensor was successfully used for the dual determination of catechol and azinphos methyl using fixed potential amperometry. Biosensor performance was optimized in terms of iridium (IV) oxide, Poly (3,4 ethylenedioxythiophene) and Tyrosinase. The surface morphology of the enzyme electrode was characterized by scanning electrochemical microscopy, atomic force microscopy, cyclic voltammetry and electrochemical impedance. Under optimized conditions, linear relationships were achieved in the range 0.05-10.65 mu M for catechol and 10-200 mu M for azinphos methyl. Limit of detection was found as 0.017 mu M for catechol and 2.964 mu M for azinphos methyl with acceptable repeatability and reproducibility. Finally, developed biosensor was successfully applied to the detection of azinphos methyl tap water, waste water, well water, human urine, serum and saliva.