The Molecular Characterization and Detection of Group A Rotavirus From Calves with Diarrhea in Turkish Republic of Northern Cyprus

ALKAN F., TİMURKAN M. Ö., KARAYEL HACIOĞLU İ.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.21, no.1, pp.127-130, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 1
  • Publication Date: 2015
  • Doi Number: 10.9775/kvfd.2014.11904
  • Journal Name: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.127-130
  • Keywords: Rotavirus, Calf, Diarrhoea, Genotype, TRNC, POLYMERASE-CHAIN-REACTION, DAIRY HERDS, GENOTYPES, BEEF, VP7
  • Ankara University Affiliated: Yes

Abstract

In this study, the molecular characterization of the Group A rotavirus obtained from calves with diarrhea in a farm in TRNC was reported. Faces samples were detected as positive by ELISA and RT-PCR depending on the amplification of the VP6 gene of rotavirus. Then the aetiological agent was analyzed by RT-PCR with generic and type-spesific to the genome segments encoding VP4 and VP7 of rotavirus. Findings revealed that the rotavirus circulating within this farm belongs to G6 and P[11] genotype. This is the first study to report the G and P genotypes of Group A rotavirus from calves with diarrhea in TRNC.