Cytotoxic effects of contemporary bulk-fill dental composites: A real-time cell analysis

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DEMİREL G., GÜR G., Demirsoy F. F., Altuntas E., Yener-Ilce B., KILIÇARSLAN M. A.

DENTAL MATERIALS JOURNAL, vol.39, no.1, pp.101-110, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.4012/dmj.2018-336
  • Journal Name: DENTAL MATERIALS JOURNAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Agricultural & Environmental Science Database, Biotechnology Research Abstracts, Communication Abstracts, Compendex, EMBASE, MEDLINE, Metadex, Civil Engineering Abstracts
  • Page Numbers: pp.101-110
  • Keywords: Bulk-fill composites, Cytotoxicity, Real-time cell analysis, iCELLigence system, HUMAN GINGIVAL FIBROBLASTS, LIGHT-CURING TIME, IN-VITRO, RESIN-COMPOSITES, MECHANICAL-PROPERTIES, LEACHABLE COMPONENTS, ELUTION, BIOCOMPATIBILITY, THICKNESS, RESPONSES
  • Ankara University Affiliated: Yes

Abstract

The aim of this study was to evaluate the cytotoxicity of contemporary flowable and paste-like bulk-fill dental composites by using a real-time cell analysis. In the present paper, cytotoxicity levels of five flowable, five paste-like bulk-fill composite materials and one conventional flowable, one conventional paste-like resin composite were examined on L929 mouse fibroblast cell line. After seeding 25,000 cells/300 mu L/well cell suspensions into the wells of an E-plate, test materials were added and observed at every 30 min intervals for 72 h. Kruskal Wallis H and Mann Whitney U multiple comparison tests were used to analyze the results. Pre-reacted glass-ionomer (PRG) containing bulk-fill composites were severely toxic at all time points (24, 48 and 72 h, p<0.05). None of the tested composites demonstrated high cell viability (>70%) at 48 and 72 h. Flowable and paste-like composites of the same brand exhibited similar cytotoxic properties (p>0.05).