Development of an In Situ Hybridization Method for Detection of Akabane Virus


Toplu N., Oğuzoğlu T. Ç., Akkoç A. N.

JOURNAL OF BIOLOGICAL METHODS, vol.11, no.1, pp.1-5, 2024 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 11 Issue: 1
  • Publication Date: 2024
  • Doi Number: 10.14440/jbm.2024.413
  • Journal Name: JOURNAL OF BIOLOGICAL METHODS
  • Journal Indexes: BIOSIS, CAB Abstracts
  • Page Numbers: pp.1-5
  • Ankara University Affiliated: Yes

Abstract

Akabane virus (AKAV) is an arbovirus belonging to the family Bunyaviridae, genus Orthobunyavirus. AKAV consists of three-segment (L, M, and S RNA segments), negative single-stranded RNA. The aim of this study was to investigate an in situ hybridization method (ISH) in a Vero E6 cell line infected with Akabane virus. The 320 base pair amplicon was obtained by RT-PCR with a primer pair and labeled with digoxigenin. Akabane virus RNAs were seen as a granular pattern in the cytoplasm of infected cells. As a result, the expression of the particular Akabane virus gene area was successfully disclosed in the current investigation using the ISH method with a digoxigenin-labeled probe.