Eletrophoretic separation of Staphylococcus aureus strains isolated from nasal carriers by means of whole-cell and filtrate protein profiles, their antibiotic susceptibilities and analysis of plasmid contents Nazal tasiyicilardan izole edilen Staphylococcus aureus suslarinin toplam hucre ve filtrat protein profillerine gore elektroforetik ayirimi, antibiyotik duyarliliklari ve plazmid iceriklerinin analizi

Sacilik S., OSMANAĞAOĞLU Ö., Cokmus C.

Mikrobiyoloji Bulteni, cilt.33, sa.3, ss.171-178, 1999 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 3
  • Basım Tarihi: 1999
  • Dergi Adı: Mikrobiyoloji Bulteni
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.171-178
  • Anahtar Kelimeler: Antibiotic resistance, Filtrate proteins, Plasmid analysis, Staphylococcus aureus, Whole cell proteins
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, 206 students from Ankara University Biology Department, were screened and 30 (14.6%) of them were found to be nasal carriers for Staphylococcus aureus. The antibiotic resistance rates of these strains were high of ampicillin and penicillin (93.3% and 90% respectively), followed by gentamicin (26.7%), sulbactam-ampicillin (20%) and methicillin (13.3%), while all strains were susceptible to vancomycin. According to plasmid analysis results, 3 different band patterns were detected. Six of the 21 beta- lactamase positive isolates showed a band of 24 kbp molecular weight which related with beta-lactamase synthesis. In sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of 30 S. aureus strains, 4 different profiles were observed by means of whole cell proteins, and these results were further confirmed and supported by SDS-PAGE of filtrate proteins.