Separation and elution order of the enantiomers of some beta-agonists using polysaccharide-based chiral columns and normal phase eluents by high-performance liquid chromatography

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Gümüştaş M., Özkan S. A., Chankvetadze B.

JOURNAL OF CHROMATOGRAPHY A, vol.1467, pp.297-305, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1467
  • Publication Date: 2016
  • Doi Number: 10.1016/j.chroma.2016.08.011
  • Journal Name: JOURNAL OF CHROMATOGRAPHY A
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.297-305
  • Keywords: Beta agonist, Enantiomer elution order, Enantioseparations, HPLC, Mobile-phase additives, Polysaccharide-based chiral columns, STATIONARY PHASES, ACIDIC ADDITIVES, BASIC DRUGS, HPLC SEPARATION, AMINO-ALCOHOLS, ENANTIOSEPARATION, CYCLODEXTRIN, EMPHASIS, CLENBUTEROL, ENANTIOSELECTIVITY
  • Ankara University Affiliated: Yes

Abstract

In this study separation of enantiomers of 8 chiral beta-agonists were studied on 6 polysaccharide-based chiral columns in polar-organic and alcohol-hydrocarbon mobile phases. No separation of enantiomers was observed on any column with polar-organic mobile phase eluents such as pure methanol, ethanol or acetonitrile. Most of the chiral analytes were resolved into enantiomers when alcohol-hydrocarbon type mobile phases were used. The most successful column was Lux Cellulose-2 on which all 8 chiral analytes were baseline resolved into enantiomers at least with one mobile phase used. The reversal of enantiomer elution order was observed dependent on the chemistry of the chiral selector and the composition of the mobile phase. (C) 2016 Elsevier B.V. All rights reserved.