Determination of hispidulin in the flowers of Inula viscosa (L.) aiton using HPLC and HPTLC methods Inula viscosa (L.) Aiton Çiçeklerinde YPSK ve YPİTK Yöntemleri ile Hispidulin Analizi

GÖKBULUT A.

Turkish Journal of Pharmaceutical Sciences, cilt.13, sa.2, ss.159-166, 2016 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 2
  • Basım Tarihi: 2016
  • Doi Numarası: 10.5505/tjps.2016.47955
  • Dergi Adı: Turkish Journal of Pharmaceutical Sciences
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.159-166
  • Anahtar Kelimeler: Hispidulin, HPLC, HPTLC, Inula viscosa
  • Ankara Üniversitesi Adresli: Evet

Özet

© 2016, Turkish Pharmacists Association. All rights reserved.The flavone hispidulin (4´,5,7-trihydroxy-6-methoxyflavone) in the flowers of Inula viscosa (L.) Aiton was quantified by using High Performance Liquid Chromatography (HPLC), and also High Performance Thin-Layer Chromatography (HPTLC) fingerprint of the methanolic I. viscosa flower extract was presented. Both methods provided a rapid and easy approach for determination of the biomarker hispidulin. In the present study HPTLC and HPLC profiles of Inula viscosa flowers were presented to detect and quantify the small flavonoid hispidulin. The results reveal that I. viscosa flowers contain serious amount (0.151± 0.007 g/100 g dry weight) of hispidulin.