Simultaneous determination of metronidazole and miconazole in pharmaceutical dosage forms by RP-HPLC

Akay C., Ozkan S. A., Senturk Z., Cevheroglu S.

Farmaco, vol.57, no.11, pp.953-957, 2002 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 57 Issue: 11
  • Publication Date: 2002
  • Doi Number: 10.1016/s0014-827x(02)01296-x
  • Journal Name: Farmaco
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.953-957
  • Keywords: reversed-phase high performance liquid chromatography, metronidazole and miconazole, pharmaceutical dosage forms, PERFORMANCE LIQUID-CHROMATOGRAPHY, DERIVATIVE SPECTROPHOTOMETRY, AMPEROMETRIC DETECTION, CREAMS, PLASMA, METABOLITES, EXTRACTION, NITRATE, DRUGS
  • Ankara University Affiliated: Yes

Abstract

A reversed-phase high performance liquid chromatography (RP-HPLC) method with UV detection is described for the simultaneous determination of metronidazole and miconazole in pharmaceutical dosage forms. Chromatography was carried out on a C18 reversed-phase column, using a mixture of methanol-water (40+60, v/v) as a mobile phase, at a flow rate of 1.0 ml min-1. Sulfamethoxazole was used as an internal standard and detection was performed using a diode array detector at 254 nm. The method produced linear responses in the concentration ranges 10-70 and 1-20 μg ml-1 with detection limits 0.33 and 0.27 μg ml-1 for metronidazole and micanozole, respectively. This procedure was found to be convenient and reproducible for analysis of these drugs in ovule dosage forms. © 2002 Éditions scientifiques et médicales Elsevier SAS. All rights reserved.