Development of a new biosensor for superoxide radicals

EMREGÜL E.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, cilt.383, sa.6, ss.947-954, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 383 Sayı: 6
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1007/s00216-005-0102-x
  • Dergi Adı: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.947-954
  • Anahtar Kelimeler: superoxide dismutase, gelatin, biosensor, free radicals, scavengers, CYTOCHROME-C, DISMUTASE ACTIVITY, PULSE RADIOLYSIS, NITRIC-OXIDE, GLUCOSE-OXIDASE, GELATIN, IMMOBILIZATION, ELECTRODE, ELECTROCHEMISTRY, OPTIMIZATION
  • Ankara Üniversitesi Adresli: Evet

Özet

A superoxide dismutase ( SOD) biosensor for determination of superoxide radicals has been developed by immobilization of superoxide dismutase within gelatin ( G) on a Pt electrode surface. The properties of the biosensor have been investigated and optimum conditions-enzyme concentration, glutaraldehyde concentration, and pH-were determined. The response of the G-SOD biosensor was proportional to O-2-(center dot-) concentration and the detection limit was 0.01 mmol L-1 at a signal-to-noise ratio of 3. The biosensor retained 89% and 60% of its sensitivity after use for three and four weeks, respectively. Immobilization of SOD on gelatin provides a biocompatible microenvironment around the enzyme and stabilizes the activity of the enzyme very efficiently. The superoxide dismutase biosensor was used to determine the antioxidant properties of acetylsalicylic acid-based drugs and the anti-radical activity of healthy and cancerous human brain tissues.