Optimization study of coumarin 7-hydroxylase enzyme activity in mouse brain and liver

Özdamar E. D., EKE B.

Fabad Journal of Pharmaceutical Sciences, vol.35, no.2, pp.77-84, 2010 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 35 Issue: 2
  • Publication Date: 2010
  • Journal Name: Fabad Journal of Pharmaceutical Sciences
  • Journal Indexes: Scopus
  • Page Numbers: pp.77-84
  • Keywords: Brain, Coumarin 7-hydroxylase, CYP450, Liver, Mouse
  • Ankara University Affiliated: Yes

Abstract

In this study, the optimum conditions (pH, amount of protein, substrate concentration, incubation time) of a cytochrome P450 dependent enzyme, coumarin 7-hydroxylase (COH), activity were determined in microsomal fractions of mouse liver and brain. It.s indicated that different reaction conditions are required for this enzyme activity in mouse liver and brain microsomes. The results obtained, reveal the requirement of optimization studies on COH activity for different tissues of mouse.