Au-Pt nanoparticles based molecularly imprinted nanosensor for electrochemical detection of the lipopeptide antibiotic drug Daptomycin

ÖZÇELİKAY G., KURBANOĞLU S., Yarman A., Scheller F. W., Ozkan S. A.

Sensors and Actuators, B: Chemical, cilt.320, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 320
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.snb.2020.128285
  • Dergi Adı: Sensors and Actuators, B: Chemical
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Analytical Abstracts, Biotechnology Research Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Anahtar Kelimeler: Molecularly imprinted polymer, Daptomycin, Platinum nanoparticles, gold nanoparticles, modified electrodes, PLATINUM NANOPARTICLES, HUMAN PLASMA, POLYMERS, SENSOR, FABRICATION, VALIDATION, URINE
  • Ankara Üniversitesi Adresli: Evet

Özet

© 2020 Elsevier B.V.In this work, a novel electrochemical molecularly imprinted polymer (MIP) sensor for the detection of the lipopeptide antibiotic Daptomycin (DAP) is presented which integrates gold decorated platinum nanoparticles (Au-Pt NPs) into the nanocomposite film. The sensor was prepared by electropolymerization of o-phenylenediamine (o-PD) in the presence of DAP using cyclic voltammetry. Cyclic voltammetry and differential pulse voltammetry were applied to follow the changes in the MIP-layer related to rebinding and removal of the target DAP by using the redox marker [Fe(CN)6]3−/4–. Under optimized operational conditions, the MIP/Au-Pt NPs/GCE nanosensor exhibits a linear response in the range of 1-20 pM towards DAP. The limit of detection and limit of quantification were determined to be 0.161pM±0.012 and 0.489pM±0.012, respectively. The sensitivity towards the antibiotics Vancomycin and Erythromycin and the amino acids glycine and tryptophan was below 7 percent as compared with DAP. Moreover, the nanosensor was also successfully used for the detection of DAP in deproteinated human serum samples.