Simultaneous LC determination of trimethoprim and sulphamethoxazole in pharmaceutical formulations

Akay C., Ozkan S. A.

Journal of Pharmaceutical and Biomedical Analysis, vol.30, no.4, pp.1207-1213, 2002 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 4
  • Publication Date: 2002
  • Doi Number: 10.1016/s0731-7085(02)00460-0
  • Journal Name: Journal of Pharmaceutical and Biomedical Analysis
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1207-1213
  • Keywords: sulfonamides, urinary tract infections, trimethoprim, HPLC, determination, PERFORMANCE LIQUID-CHROMATOGRAPHY, SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION, REVERSED-PHASE, HUMAN SERUM, URINE, SULFONAMIDES, TABLETS, HYDROCHLOROTHIAZIDE, PLASMA
  • Ankara University Affiliated: Yes

Abstract

In the present study, a simple, sensitive, precise and rapid reversed-phase high performance liquid chromatographic (HPLC) method with ultraviolet detection for the simultaneous analysis of trimethoprim (TMP) and sulphamethoxazole (SPM) is developed and applied to the determination in commercial pharmaceutical preparations. These compounds are well separated on a Bondapak C18 reverse phase column using a mobile phase consisted of a mixture of methanol:water (60:40; v/v) adjusted to pH 3 with 10% orthophosphoric acid at a flow rate of 1.8 ml min-1. The proposed method was linear in the range 2.0-10.0 μg ml-1 for TMP and 10.0-50.0 μg ml-1 for SPM. The limit of detection were 0.45 and 1.21 μg ml-1 for TMP and SPM, respectively. The method which is rapid, simple and does not require any separation step, has been successfully applied to the assay of commercial tablet and oral suspension dosage forms containing TMP and SPM. © 2002 Elsevier Science B.V. All rights reserved.