Determination of clenbuterol HCI in human serum, pharmaceuticals, and in drug dissolution studies by RP-HPLC

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Ozkan Y., Ozkan S. A., Aboul-Enein H.

Journal of Liquid Chromatography and Related Technologies, vol.24, no.5, pp.679-691, 2001 (SCI-Expanded)

• Publication Type: Article / Article
• Volume: 24 Issue: 5
• Publication Date: 2001
• Doi Number: 10.1081/jlc-100103403
• Journal Name: Journal of Liquid Chromatography and Related Technologies
• Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Page Numbers: pp.679-691
• Ankara University Affiliated: Yes

Abstract

A simple, reliable, and rapid RP-HPLC method has been developed for the determination of Clenbuterol HC1 in human serum and pharmaceuticals, in order to carry out drug dissolution studies for clenbuterol tablets. This compound is well separated on a C18 column by using the mobile phase consisting of a mixture of acetonitrile and ion-pair buffer (32:68; v/v) at a flow rate of 1.5 mL/min. Detection was carried out using a UV detector at 244 nm. ephedrine HC1 was used as an internal standard. Minimum detection limit obtained was 3.78 ng/mL. This method was applied, without any interferences from the excipients, for the determination of the drug in tablet formulation, human serum, and drug dissolution studies.