Akademik Veri Yönetim Sistemi
13th International Drug Chemistry Conference (13th DCC), Antalya, Türkiye, Antalya, Türkiye, 6 - 09 Şubat 2025, ss.255
Varroa destructor, a prevalent pest of honeybees, is a leading cause of global bee population decline.
The acaricides available for controlling this parasite are limited, with flumethrin, a pyrethroid, widely used
to combat Varroa destructor. However, the excessive and improper use of flumethrin has raised serious
concerns, as it induces toxicity in honeybees and accumulates in bee-derived products due to its
lipophilic nature. This residue accumulation compromises the quality of bee products and poses notable
risks to public health, including fatalities associated with pyrethroid toxicity1. Therefore, detecting
flumethrin residue is critical, but the available detection methods are limited. This study aims to develop
a fast, selective, and sensitive method for the detection of flumethrin using high-performance liquid
chromatography with ultraviolet detection.
A rapid liquid-liquid extraction method using hexane was developed for flumethrin detection in honey
and plasma samples. The samples were adjusted to pH 11, with NaCl added to enhance ionic strength,
followed by vortexing and centrifugation. The extracts were evaporated, reconstituted in acetonitrile, and
injected directly into an HPLC system. Extraction efficiency was determined as 82.76% and extraction
process took 10 minutes with this method.
Chromatographic separation was achieved using a Kinetex EVO C18 column, with a mobile phase of
0.1% acetic acid and acetonitrile. Detection was performed at a wavelength of 267 nm, and samples
were injected at a volume of 20 μL. A rapid analysis method was developed with a total run time of under
10 minutes and a retention time of 6.5 minutes. The method was validated in accordance with the
guidelines set by the International Council for Harmonization. The method's limits of detection and
quantification were determined using signal-to-noise ratios, with values of 0.03 μg/mL and 0.05 μg/mL,
respectively.
The developed extraction and determination method offers a reliable and selective approach for the