DETERMINATION OF DIHYDROERGOTAMINE VIA LC METHOD COUPLED WITH FLUORESCENCE DETECTION IN PHARMACEUTICAL FILM PREPARATIONS

KURBANOĞLU S., Esim O., Ozkan C. K., Savaser A., Ozkan Y., ÖZKAN S. A.

REVUE ROUMAINE DE CHIMIE, vol.62, no.6-7, pp.517-523, 2017 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 62 Issue: 6-7
  • Publication Date: 2017
  • Journal Name: REVUE ROUMAINE DE CHIMIE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.517-523
  • Keywords: chromatography, determination, dihydroergotamine maleate, fluorescence, LIQUID-CHROMATOGRAPHY, TRIPTAN THERAPY, MIGRAINE, MESYLATE, SAFETY, PLASMA, PHARMACOKINETICS, PREVALENCE, ERGOTAMINE, EFFICACY
  • Ankara University Affiliated: Yes

Abstract

In this study, Dihydroergotamine Maleate was determined by LC coupled with fluorescence detector, using Agilent Eclipse XDB-C8 5 mu m 4.6 x 150 mm column with a flow rate of 1.2 mL/min, at 25 degrees C with mobile phase composition of 70:30 Buffer (containing 1.5% Formic Acid): ACN at an excitation wavelength of 280 nm and an emission wavelength of 350 nm. The analysis of Dihydroergotamine mesylate was obtained within 5 min. Sensitive determination method for the dihydroergotamine mesylate with LC coupled with fluorescence detector was achieved in range of 8.56 x10(-7) to 1.71x10(-4) M with LOD value of 4.48x10(-8) M and LOQ value of 1.36x10(-7) M. The proposed method has been extensively validated in terms of precision, accuracy. Linear range, limit of detection and quantification values, are also calculated and discussed according to ICH Guidelines and USP criteria. The method is also used for the determination Dihydroergotamine in pharmaceutical film preparations and Dihydroergotamine was obtained from film formulations with an acceptable recovery results.