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BELDER N., Coskun Ö., DOĞANAY ERDOĞAN B., İLK DAĞ Ö., SAVAŞ B., ENSARİ A., ...More
Pathology, research and practice, vol.212, no.8, pp.678-85, 2016 (SCI-Expanded)
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Publication Type:
Article / Article
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Volume:
212
Issue:
8
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Publication Date:
2016
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Doi Number:
10.1016/j.prp.2015.11.008
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Journal Name:
Pathology, research and practice
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Journal Indexes:
Science Citation Index Expanded (SCI-EXPANDED), Scopus
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Page Numbers:
pp.678-85
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Keywords:
FFPE tissues, RNA isolation, Gene expression, Microarray, PARAFFIN-EMBEDDED TISSUES, GENE-EXPRESSION ANALYSIS, OLIGONUCLEOTIDE MICROARRAYS, EXTRACTION METHODS, ARCHIVAL MATERIAL, FROZEN TISSUE, NUCLEIC-ACIDS, TUMOR SAMPLES, DEGRADED RNA, CANCER
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Ankara University Affiliated:
Yes
Abstract
Background: Genome-wide gene expression profiling analysis of FFPE tissue samples is indispensable for cancer research and provides the opportunity to evaluate links between molecular and clinical information, however, working with FFPE samples is challenging due to extensive cross-linking, fragmentation and limited quantities of nucleic acid. Thus, processing of FFPE tissue samples from RNA extraction to microarray analysis still needs optimization.