From RNA isolation to microarray analysis: Comparison of methods in FFPE tissues.

BELDER N., Coskun Ö., DOĞANAY ERDOĞAN B., İLK DAĞ Ö., SAVAŞ B., ENSARİ A., ...Daha Fazla

Pathology, research and practice, cilt.212, sa.8, ss.678-85, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 212 Sayı: 8
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.prp.2015.11.008
  • Dergi Adı: Pathology, research and practice
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.678-85
  • Anahtar Kelimeler: FFPE tissues, RNA isolation, Gene expression, Microarray, PARAFFIN-EMBEDDED TISSUES, GENE-EXPRESSION ANALYSIS, OLIGONUCLEOTIDE MICROARRAYS, EXTRACTION METHODS, ARCHIVAL MATERIAL, FROZEN TISSUE, NUCLEIC-ACIDS, TUMOR SAMPLES, DEGRADED RNA, CANCER
  • Ankara Üniversitesi Adresli: Evet

Özet

Background: Genome-wide gene expression profiling analysis of FFPE tissue samples is indispensable for cancer research and provides the opportunity to evaluate links between molecular and clinical information, however, working with FFPE samples is challenging due to extensive cross-linking, fragmentation and limited quantities of nucleic acid. Thus, processing of FFPE tissue samples from RNA extraction to microarray analysis still needs optimization.