A Novel Enzymatic Biosensor for the Detection of Catechol Using Multi-walled Carbon Nanotubes and Gold Nanowires

KURBANOĞLU S., ÖZKAN S. A.

ELECTROCATALYSIS, cilt.9, sa.2, ss.252-257, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 9 Sayı: 2
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s12678-017-0408-4
  • Dergi Adı: ELECTROCATALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.252-257
  • Anahtar Kelimeler: Catechol, Carbon nanotubes, Enzymatic biosensors, Gold nanowire, Tyrosinase, TYROSINASE, NANOPARTICLES, ELECTRODES
  • Ankara Üniversitesi Adresli: Evet

Özet

Catechol biosensing with a low limit of detection is an essential topic for researchers due to its oxidizable properties that can easily be monitored amperometrically. Screen-printed electrodes are disposable devices that have found a widespread use in the field, including analytical chemistry, drug control, and clinical and environmental analysis, particularly with the significant developments that have taken place over the last few decades. This study proposes a novel catechol biosensing platform based on the synergetic effect of the multi-walled carbon nanotube, gold nanowires, and the tyrosinase enzyme. All the parameters affecting the biosensing response including the amounts of gold nanowire and tyrosinase were optimized and the method was further validated. Using the synergetic effects of the multi-walled carbon nanotube and gold nanowires, the limits of detection and quantification values for the sensitive determination of catechol were found to be 0.027 and 0.080 mu M, respectively.