Akademik Veri Yönetim Sistemi
TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES, cilt.20, sa.3, ss.141-148, 2023 (ESCI)
Objectives: Trachystemon orientalis (L.) G. Don, colloquially known in Türkiye as “kaldırık”, is an edible plant belonging to the Boraginaceae. This
plant has been practiced in traditional medicine for many years for its various therapeutic benefits. The effectiveness and chemical composition of
plants can vary depending on their parts, age, and extraction solvent. Therefore, the current study aimed to define the biological activities of various
parts and extracts of T. orientalis, which were collected in distinct seasons as young and mature, and investigate the main component responsible
for these biological effects.
Material and Methods: Plant material was collected in different seasons from the northwest of Türkiye. 2,2’-Azinobis-(3-ethylbenzothiazoline-6-
sulphonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities were investigated to assess antiradical and
antioxidant potential of the extracts. Anti-inflammatory activity of the extracts was also tested using human red blood cell membrane stabilizing
method. Folin-Ciocalteu test was conducted to determine the total phenolic content. Reverse phase-high performance liquid chromatographyphotodiode array detector (RP-HPLC-PDA) analysis was performed.
Results: Both methanol and aqueous extracts exhibited significant radical scavenging and anti-inflammatory activities compared with control
(p<0.05). The highest percentage of inhibition on ABTS and DPPH free radicals was obtained in aqueous extracts of the mature herbs and roots,
respectively. Methanol extracts of the mature roots and herbs exhibited the strongest anti-inflammatory capacity. Rosmarinic acid possessed a
much higher antioxidant and anti-inflammatory effect than the reference compounds used in each assay in our study. High rosmarinic acid content
of the extracts suggests that the compound responsible for the great biological activity potential is rosmarinic acid.
Conclusion: To the best of our knowledge, the presence of rosmarinic acid in herbs and roots of T. orientalis was shown for the first time in our
present study. Phytochemical composition and effective biological activities of T. orientalis explain its traditional use and indicate its significant
potential in pharmaceutical industry applications.
Key words: Antioxidant activity, anti-inflammatory activity, rosmarinic acid, RP-HPLC-PDA, Trachystemon orientalis