Strategies for improving the functionality of an affinity bioreactor

Wang T., Yang Z., EMREGÜL E., David A., Balthasar J., Liang J., ...More

INTERNATIONAL JOURNAL OF PHARMACEUTICS, vol.306, no.1-2, pp.132-141, 2005 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 306 Issue: 1-2
  • Publication Date: 2005
  • Doi Number: 10.1016/j.ijpharm.2005.09.011
  • Journal Name: INTERNATIONAL JOURNAL OF PHARMACEUTICS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.132-141
  • Keywords: protamine bioreactor, heparin adsorption, Langmuir adsorption isotherm, poly(ethylene glycol) modification, polylysine amplification, BIOANALYTICAL APPLICATIONS, PROTAMINE, HEPARIN, MEMBRANE, IMMOBILIZATION, PROTEINS
  • Ankara University Affiliated: Yes

Abstract

Heparin employed in extracorporeal blood circulation (ECBC) procedures (e.g. open heart operations) often leads to a high incidence of bleeding complications. Protamine employed in heparin neutralization, on the other hand, can cause severe adverse reactions. We previously developed an approach that could prevent both heparin- and protamine-induced toxic side effects concomitantly. This approach consisted of placing a hollow fiber-based bioreactor device containing immobilized protamine (termed a "protamine bioreactor") at the distal end of the ECBC procedure. This protamine bioreactor would remove heparin after heparin served its anticoagulant purpose in the ECBC device, thereby eliminating heparin-induced bleeding risks. In addition, this protamine bioreactor would prevent prolamine from entering the patients, thereby aborting any protamine-induced toxic effects. Both in vitro and in vivo studies have successfully demonstrated the feasibility of this approach.