Quartz tuning fork biosensor: A potential tool for SARS-CoV-2 detection

Kavacık M., İnce B., ARI F., ÜNAL M. A., SEZGİNTÜRK M. K., ÖZKAN S. A.

MICROCHEMICAL JOURNAL, vol.195, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 195
  • Publication Date: 2023
  • Doi Number: 10.1016/j.microc.2023.109498
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Food Science & Technology Abstracts, Index Islamicus, Veterinary Science Database
  • Keywords: Biosensor, COVID-19, Immunosensor, QTF, Quartz Tuning Fork, SARS-CoV-2
  • Ankara University Affiliated: Yes


Portable, fast, and practical devices are important in the fight against epidemic diseases. The lack of readily available small-scale commercial solutions prompted us to delve deeper into this research effort. Investment in Quartz Tuning Fork (QTF)-based biosensor research is significant due to its seamless integration with miniaturised and portable devices. Within the scope of this study, a new, cost-effective, and versatile frequency analyser designed for detecting diseases, compatible with screens of different sizes, has been introduced. The modified QTF sensor occured mass-sensitive SARS-CoV-2 nucleocapsid protein (SARS-NP) detection with remarkable simplicity, sensitivity, and selectivity. The developed QTF-based biosensor has an impressive linear detection range ranging from 5 to 200 ng/mL and an exceptionally low limit of detection (LOD) value of 1.72 ng/ mL. The sensitivity of the developed QTF-based biosensor was obtained as 50 Hz/1 mu M. The biosensor response was then evaluated in commercial human serum sample. After 15 days of storage, these biosensors retained approximately 93.7 % of their initial activity. Additionally, a Scanning Electron Microscope (SEM) and Atomic Force Microscope (AFM) comprehensively characterised the electrode surface.