Akademik Veri Yönetim Sistemi
Fabad Journal of Pharmaceutical Sciences, cilt.37, sa.4, ss.191-196, 2012 (Scopus)
In recent years, some studies have shown that CYP2E1 enzyme is associated with many diseases. Determining the activity of this enzyme will help to obtain new clues about several mechanisms in unknown progression of diseases. The purpose of this study is to assess the optimal conditions for CYP2E1 activity. In this context, the optimum conditions (amount of protein, substrate concentration and incubation time) were investigated in C57Bl/6 mouse brain and liver microsomes. In this study, it was found out that, different reaction conditions are required for this enzyme activities in microsomal brain and liver CYP2E1 of C57Bl/6 mice. According to our research results, these optimization studies should be examined for CYP2E1 activity in different tissues of mouse for future investigation.