Electrochemical Study of the Interaction Between the Antibacterial Drug Gemifloxacin and dsDNA Using Pencil Graphite Electrode

BOZAL PALABIYIK B., Topal B., USLU B., ÖZKAN S. A., Aboul-Enein H. Y.

CURRENT ANALYTICAL CHEMISTRY, vol.8, no.4, pp.528-533, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 4
  • Publication Date: 2012
  • Doi Number: 10.2174/157341112803216735
  • Journal Name: CURRENT ANALYTICAL CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.528-533
  • Keywords: Biosensor, dsDNA, Voltammetry, Guanine, Gemifloxacin, CALF THYMUS DNA, BIOSENSORS, NORFLOXACIN, BINDING
  • Ankara University Affiliated: Yes

Abstract

Gemifloxacin (GMF) is a broad-spectrum quinolone antibacterial agent used in the treatment of acute bacterial exacerbation of chronic bronchitis and mild-to-moderate pneumonia. The investigation of the GMF - double-stranded DNA (dsDNA) interaction using an electrochemical DNA-biosensor, showed for the first time clear evidence of interaction with dsDNA. The interaction between GMF and dsDNA onto pencil graphite electrode (PGE) was assessed at pH 4.80 acetate buffer using differential pulse voltammetry (DPV). The interaction mechanism was disclosed with the decrease of the guanine oxidation peak current. The changes in the experimental parameters such as the accumulation time and the concentration of GMF were studied. Linearity of the guanine oxidation signals was obtained in response to the range of 0.5 - 8.0 mu g/mL GMF. Detection and quantification limits in the range of mu g/mL were determined.