Ultrasensitive electrochemical immunosensor system for determination of autologous SOX2 antibody

Özçelikay-Akyıldız G., ÜNAL M. A., Atakan Ş., Gülden S., Kızılelma B., Aydın S., ...More

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, vol.241, 2024 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 241
  • Publication Date: 2024
  • Doi Number: 10.1016/j.jpba.2024.115992
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, International Pharmaceutical Abstracts, Veterinary Science Database
  • Keywords: Electrochemical sensor, Immunosensor, autologous SOX2 antibody, Sensitivity
  • Ankara University Affiliated: Yes


Lung cancer is mainly seen as the cancer type in the world. Lung cancer causes the death of many people. It is classified as large -cell neuroendocrine carcinoma (LCNEC), small -cell lung cancer (SCLC), and adenocarcinoma by the World Health Organization (WHO) in 2015. Small cell lung cancer (SCLC) is a highly aggressive type of cancer, accounting for approximately 20% of all cases. By performing the serological analysis of expression cDNA libraries (SEREX), the humoral immune response of SCLC patients is determined. SEREX of SCLC cell lines using pooled sera of SCLC patients led to the isolation of SOX2 genes. The between SOX2 antigen expression intensity and autologous antibody presence has a significant correlation because SOX2 is the main antigen eliciting antiSOX responses. Electrochemical biosensors take much attention because of their simplicity, selectivity, and sensitivity in clinical analysis. Antibody -based surface recognizes antibody -specific antigens. This work aims to fabricate an immunosensor for determining autologous SOX2 antibodies using a multi -walled carbon nanotubemodified screen -printed electrode (DRP-MWCNT). All immobilization processes were evaluated with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The critical parameters were optimized, such as EDC/NHS concentration and time, SOX2 protein concentration and incubation time, BSA ratio, BSA blocking time, and anti-SOX2 antibody incubation time. The developed immunosensor, under optimal conditions, shows a linear response of autologous SOX2 antibody between 0.005 ng.mL-1 and 0.1 ng.mL-1. The limit of detection and quantification were 0.001 and 0.004 ng.mL-1, respectively. The electrode morphologies were examined with a scanning electron microscope (SEM). Lastly, the developed immunosensor was applied to a synthetic serum sample, and the linear range was compared with enzyme -linked immunosorbent assay (ELISA).